Improved Dual Color Standards are brighter than the competition on both gels and blots. Increased overall brightness provides visual robustness throughout the entire protein electrophoresis and western blotting workflow. 8 blue-stained bands and 2 pink reference bands (25 and 75 kD).Mixture of 10 recombinant proteins of precise molecular weights (10–250 kD).
#BIO RAD WESTERN BLOT ALL BLUE STANDARDS PLUS#
Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. Details of Bio-Rads protein standards are provided in the tables and figures below.
#BIO RAD WESTERN BLOT ALL BLUE STANDARDS FULL#
LC-MS innovation: Improve analytical accuracy Bio-Rad offers a full range of prestained and unstained standards for various applications. The gel was then transferred to an Immun-Blot PVDF membrane (Bio-Rad. Which microbioreactor modules are best for you All samples were run in duplicate and normalized to total protein levels (in mg). Which solid support is right for your oligo synthesis? Lab Automation, AI & Digital Transformation.Food Contamination: Quality, Safety & Authenticity.The Immun-Star WesternC kit allows researchers to take full advantage of the features offered by their imaging instruments. The Immun-Star WesternC kit generates an intense signal immediately that is easily captured and repeatedly imaged by CCD cameras such as Bio-Rad's Molecular Imager VersaDoc and ChemiDoc XRS Systems. To utilise and complement the Precision Plus Protein WesternC standards, the Immun-Star WesternC kit is specifically designed for CCD imagers. Optimized for CCD imagers, the Precision Plus Protein WesternC standards are the sharpest in the industry and ideal for visualization of blots, production of publication-quality images of bands on blots, and accurate molecular weight estimation direction from acquired blot images. Transblot Electrophoretic System, use the Bio-Rad instructions for those. V3Western Workflow Save time, identify problems earlier, and have more confidence in your western blot results. The 25, 50 and 75kD bands are stained pink for easy band reference and blot orientation, with all other bands prestained blue.Įach standard has an integral Strep-tag sequence to enable parallel detection on western blots using StrepTactin-HRP conjugates, also provided by Bio-Rad. 2X Treatment Buffer + MCE + Bromophenol Blue, pH 6.8. The Precision Plus Protein WesternC standard offers ten prestained protein bands over the same broad range as all Precision Plus Protein standards, ie, from 10 to 250kD. This allows users to monitor electrophoresis progress, assess transfer efficiency and direct visualisation of the WesternC standards through Western blotting. The Precision Plus Protein WesternC Standard.Īpplicable for all 1D gel electrophoresis experiments, the Precision Plus Protein WesternC Standard combines the convenience of prestained bands with western blot detection. The complementing Immun-Star WesternC kit will further establish Bio-Rad as a leading provider of protein standards and allow users to visualise the standards on CCD imagers, it says. The Immun-Star WesternC kit is a complementary chemiluminescent detection kit that is optimised for CCD imaging of the WesternC standards and protein samples.Ĭurrently, Bio-Rad offers a sample size of both the WesternC standard and the Immun-Star WesternC kit.īio-Rad envisions that the Precision Plus Protein WesternC standard will become the leading western blotting standard in all areas of biological research. The Precision Plus Protein WesternC standards offers ten prestained protein bands that can also be detected through chemiluminescent western blotting. Bio-Rad Laboratories has announced the release of a broad-range, prestained chemiluminescent protein standard and a chemiluminescent detection kit for protein sizing and expression analysis.
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